The BFF has a long history in the production of thermophiles (see list at the bottom of this post). Sterilize in place fermentors are well suited for production of these organisms since they require temperatures sometimes over 100 °C. Autoclavable vessels can’t reach more than 65 °C in most cases. Thermophiles have the added challenge of requiring anaerobic culture conditions. Thermus thermophilus is commonly produced at the BFF and we were contracted by Venki Ramakrishnan for this strain which he used for purification of the ribosome. Dr. Ramakrishnan was awarded the Nobel Prize in Chemistry for determining the structure of the ribosome.
BFF is proud to exhibit at the Society for Industrial Microbiology's Symposium for Biotechnology for Fuels and Chemicals to be held 28April - 30April 2015
This is a post-doctoral position within the Bioexpression and Fermentation Facility focusing on microbial fermentation. Qualified applicants can apply through the UGA Job Site (note - you may need to use your browser back button after clicking the link then click the link a second time to access the job posting)
This position has the following job duties:
- Conduct laboratory and pilot-scale experiments (up to 800L)
- Organize and maintain the fermentation development lab
- Identify, analyze and troubleshoot fermentation problems
- Develop, maintain and revise fermentation SOPs
The Bioexpression and Fermentation Facility (BFF) is accepting applications for a Research Professional level position in the protein purification group. The job responsibilities are as follows:
- Executing small and large-scale purification projects
- Running FPLC and HPLC chromatography systems for multi-mg or gram quantities of proteins
- Familiarity with TFF systems is also desirable as well as the ability to troubleshoot chromatography equipment issues as needed
- Write reports based on data collected from experiments according to facility guidelines and/or client specifications
- ... Read Full Article
The Masters in Biomanufacturing and Bioprocessing program is pleased announce the graduation of the latest group of students. The students completed their training which included intensive study in microbial physiology, fermentation as well as business classes alongside MBA students in the Terry College of Business. The students also complete an internship with this group interning at Merial and the BFF.
The MBB program trains students to take on leadership roles in biotechnology having gained knowledge in diverse areas such as fermentation, cell culture, regulatory affairs and finance. As part of the program students get hands on experience with equipment used in industry and develop business skills that will assist them in their future endeavors. We are very proud of our graduating students and are excited for our current students who will soon enter the growing biotechnology field.
Siran along with her advisor, Dr. Scott Merkle, previously worked with BFF on a project featured in the columns article linked below focused on production of sweetgum embryos. Her worked has helped us better uderstand how fermentors can be used for production of embryonic plant tissue. Siran has several articles in the works with at least one to include BFF staff member Paul Volny (pictured above behind Dr. Merkle (left) and Siran). Congratulations Siran!
The BFF was established in 1967 and is one of the largest fermentation pilot plants in the... Read Full Article
The Bioexpression and Fermentation Facility is pleased to announce that Dr. Michelle Lewis will join the facility as Assistant Director next month. She will focus on BFF's cell culture services, improving our quality system and training staff and students. Dr. Lewis received two B.S. degrees in Biochemistry and Molecular Biology from Western Kentucky University and her Ph.D. in Biochemistry from the University of Georgia. Previously, Dr. Lewis worked as co-director of the Vanderbilt High-throughput Screening Facility. The lab is a multidisciplinary core facility that provides screening and automation services to develop and support basic and applied research focused on small molecule pharmacology. During her time there, the facility boasted many successful projects and collaborations. Her responsibilities included supervising the staff and projects, finding and... Read Full Article
In a multi-year grant supported project, researchers in the Hahn Lab at the Complex Carbohydrate Research Center and the BFF have generated a panel of monoclonal antibodies against a variety of plant cell wall targets. Two of these antibodies are the subject of work to be presented at Plant Biology 2014. Please see abstract details below.
S-Lignin-Directed Monoclonal Antibodies as Probes for Studies of Lignin Structure, Deposition, and Dynamics
Author(s): Claudia L. Cardenas - University of Georgia (Role: Author)Utku Avci - Complex Carbohydrate Research Center, University of Georgia (Role: Author) Ruth Davis - University of Georgia (Role: Author) Ruihua Dong - University... Read Full Article
Finding the right antibody can be difficult because a high quality antibody cannot be identified based on existing information. Depending on the target, there may be a lot of choices, but not a lot of information on how well the antibody worked or the information may not be independently verified. Science Exchange has established an Antibody Validation Program to test different antibodies in order to determine how well they work in western blot applications. BFF has developed a partnership with Science Exchange for validation of antibodies and will use the LICOR platform for visualization of blots. "We are excited about this new series of projects with Science Exchange that builds on the experience of our monoclonal antibody team” said Dr. David Blum, Director of BFF.
For more information about the antibody validation program visit... Read Full Article
The UGA Technology Commercialization Office has established a master licensing agreement with KeraFast for marketing research materials generated on campus including antibodies developed at BFF. Please see the full article.
The BFF was established in 1967 and is one of the largest fermentation pilot plants in the United States offering services to universities, government agencies and industry. The facility has four focus areas which include Fermentation, Protein Purification, Cell Culture and Monoclonal Antibodies. The BFF has 26 bioreactors ranging... Read Full Article