Efraim Racker said "don't waste clean thinking on dirty enzymes." Sometimes tagging a protein with histidines isn't good enough with respect to protein purity. The BFF has experience with protein expression, purification and polishing to get your protein prep as "clean" as it can be. We can use our suite of fermentors & bioreactors to produce your protein of interest typically at higher expression levels than shake flasks, then use our automated equipment to process the expressed protein. The following is a typical process work-flow:
Stage 0 Process Development
The BFF has small-scale fermentors where we can test different conditions to come up with the optimal process for your construct.